Mycoplasma is a unique genus of bacteria. Unlike most bacterial species, Mycoplasma lacks a cell wall allowing it to assume diverse shapes. These characteristics challenge the effectiveness of antibiotics in cell culture media, as many antibiotics target the bacterial cell wall. Mycoplasma, among the tiniest bacteria (0.2–0.8 µm in size), presents a stealthy contamination risk.
How can Mycoplasma contamination impact cell culture behaviour?
Contamination of cell cultures by Mycoplasma can impact cell behaviour, rendering experimental results unreliable and irreproducible. Detecting Mycoplasma proves challenging due to its small size, potentially affecting cultured cells for extended periods and undermining prior research outcomes. Mycoplasma species commonly disrupt protein, RNA, and DNA synthesis through nutrient scarcity, alter cellular morphology, and modulate cytokine expression, predominantly with negative consequences. Various other detrimental effects may also arise.
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Why test for Mycoplasma contamination?
Removing Mycoplasma from cell cultures is possible but often time-consuming, sometimes taking weeks or even longer. In many cases, it’s more practical to discard the affected culture. However, early detection simplifies the process. Certain antibiotics targeting DNA replication or protein expression can eliminate Mycoplasma when present at low levels. Regular testing enables the rescue of valuable or limited-resource cell cultures.While some changes in cell cultures hint at Mycoplasma contamination, the only definitive method of detection is specific testing.
How can we help?
Biofortuna have thirty years’ of experience of Cell line QC services within our Group. We offer a fast and efficient service tailored to suit your needs.
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