Cell line authentication

Quick, simple and accurate Cell line authentication in as little as three days

Using highly sensitive Short Tandem Repeats (STRs), our PCR and sequencing analysis allows for high level discrimination between cell sets, samples and cultures. STR analysis is the gold standard for Cell Line Authentication services.

With over thirty years of experience, we offer a comprehensive service designed to give you complete confidence in your models, whilst saving precious time with simple ordering, convenient sample collection and prompt reporting in as little as three days.

Simple ordering process

3-day turnaround

UK based

Ambient sample collection

Competitive pricing

Our Cell line authentication service features:

DNA Analysis using the Promega® PowerPlex Fusion system, containing 22 STR loci plus two gender defining markers.
Fast turnaround: Results issued in as little as three working days of our laboratory receiving your samples.
Completely confidential
Simple sample collection process using cards provided
Mycoplasma detection and mixture analysis available
Electropherograms provided as standard
Reference profiling against public databases where required & full result interpretation
Performed under our ISO 17025 accredited procedures

We offer analysis of 24 polymorphic STRs which allows cross identification of cell lines with an extremely high power of discrimination. The loci which will be tested include the 17 markers contained within ICLAC recommended databases in compliance with ASN-0002-2022.

By analysing these markers, each sample can be matched and verified with a high degree of precision. The allelic data from samples can be compared against each other, a known reference or verified against cell line databases.

Any sample(s) which require repeat testing will be communicated to the sender as soon as possible, to prevent delay. In a small number of instances, it may not be possible to complete the analysis, most often due to poor DNA quality. All unsuccessful samples are retested once more. Charges for all tests will still apply where it is not possible to issue a result.

If you prefer to compare the data yourself, we can send the raw files/exported genotype data as is your preference.

Cell line authentication in the lab
Cell Line Authentication

Simple, straightforward collection

Our Cell Line Authentication service includes DNA extraction of cell cultures spotted onto our bespoke sample collection kits. We are also able to work with frozen cell pellets at a minimum yield of 2 x 105 cells.

We are also able to work with pre-extracted DNA samples of a minimum concentration of 10ng/µl in a total volume of 20µl. DNA samples must be free of any PCR inhibitors such as EDTA, proteins, polysaccharides or DMSO.

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