Loop-mediated isothermal amplification (LAMP)

Biofortuna has extensive experience in the development and manufacture of LAMP assays.

We can work with you throughout the product development cycle, from initial assay design through to full development and validation.

What is a LAMP assay?

Loop-mediated isothermal amplification (LAMP) is a single-tube technique for the amplification of DNA. Reverse Transcription Loop-mediated isothermal Amplification (RT-LAMP) combines LAMP with a reverse transcription step to allow the detection of RNA.

In contrast to the polymerase chain reaction (PCR) technology, in which the reaction is carried out with a series of alternating temperature steps or cycles, isothermal amplification is conducted at a constant temperature which does not require the need for a thermal cycler.

Advantages

Amplification of DNA takes place at an isothermal condition (63 to 65°C) with greater efficiency.
Thermal denaturation of double stranded DNA is not required.
LAMP helps in specific amplification as it designs 4 primers to recognize 6 distinct regions on the target gene.
LAMP is cost effective as it does not require special reagents or sophisticated equipment.
This technology can be used for the amplification of RNA templates in presence of reverse transcriptase.
LAMP assay takes less time for amplification and detection.

Applications

LAMP assays have been designed for the detection of a wide range of RNA and DNA targets from all manner of sample types for point-of-care (POC) and field diagnostics.

LAMP can be used to detect viral, bacterial and parasitic diseases such as such as tuberculosis (TB) malaria and SARS-CoV-2

Do you have a project that we can help you with? Contact us now to arrange a meeting