Extraction and downstream analysis

We provide a range of extraction services to leading pharmaceutical and clinical research organisations, academic and research institutions.

Our range of services is varied and includes DNA, RNA and miRNA extraction across several hundred clinical trials as well as less complex extraction only services or extraction combined with additional downstream services.

DNA Extraction

We provide a rapid and affordable DNA extraction service which generates high quality, high yield DNA. Using a range of validated procedures such as Qiagen, PAXgene, MagMax and OMEGA extraction kits, we are able to extract DNA from a broad range of biological matrices including:

Whole blood
Formalin Fixed Paraffin Embedded Tissue (FFPE)
Lymphocytes, buffy coats, plasma and sera
Saliva collected into commercially available kits for e.g. Oragene tubes
Frozen tissue and biopsy material
Buccal scrapes / swabs
Cultured cells (mammalian and bacterial)
Blood cards
Bone samples

As part of our services portfolio we also offer a rapid turnaround DNA extraction service

RNA/miRNA

We have proven experience in RNA/miRNA isolation from a number of different sample types using validated procedures such as TriZol, Qiagen, PAXgene, MagMax, ReliaPrep, OMEGA extraction kits. Sample types include:

Formalin Fixed Paraffin Embedded Tissue (FFPE)
Whole blood (including samples collected into PAXgene/Tempus tubes)
Leukocytes collected into LeukoLOCK™ tubes
Plasma and sera
Saliva
Frozen tissue and biopsy material
Cultured cells (mammalian and bacterial)
Cultured cells (mammalian and bacterial)

We also offer a number of associated QC options including agarose gel electrophoresis.

Quantification and Quality Assessment

We offer a range of nucleic acid quantification options:

PicoGreen assay (DNA)
RiboGreen assay (RNA)
Spectrophotometry/absorbance measurement (DNA and RNA)
qPCR (RNaseP Analysis)

We also offer a number of associated qualitive analysis options including:

Bioanalyzer analysis for DNA, RNA and miRNA
Fragmentation analysis by agarose gel electrophoresis
Purity ratio assessment by OD measurement
The three pillars of the Biofortuna service
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