DNA / RNA extraction

We provide DNA, RNA and miRNA extraction services to leading pharmaceutical and clinical research organisations, academic and research institutions.
We have extensive experience in providing regulatory compliant extraction services in several hundred clinical trials.

We offer an extraction-only service or extraction combined with downstream services.

Since 1999 our proprietary Nucleon extraction chemistry has been used for rapid and efficiently extraction of high yields of pure, high molecular weight DNA. Nucleon is compatible for use with whole blood and other sample types such as tissue and saliva.

DNA Extraction

We provide a rapid and affordable DNA extraction service which generates high quality, high yield DNA. Using our proprietary Nucleon DNA extraction chemistry and other validated procedures (such as Qiagen, PAXgene, MagMax, OMEGA extraction kits) we are able to extract DNA from a broad range of biological matrices including:

Whole blood
Formalin Fixed Paraffin Embedded Tissue (FFPE)
Lymphocytes, buffy coats, plasma and sera
Saliva collected into commercially available kits for e.g. Oragene tubes
Frozen tissue and biopsy material
Buccal scrapes / swabs and mouthwash samples
Cultured cells (mammalian and bacterial)
Blood cards

As part of our services portfolio we also offer a rapid turnaround DNA extraction service

Nucleon

The Nucleon extraction chemistry rapidly and efficiently extracts high yields of pure, high molecular weight DNA. Nucleon is compatible for use with whole blood and other sample types such as tissue and saliva.

The DNA is not bound to a solid surface at any point in the process. This avoids exposing the DNA to shearing forces and promotes the recovery of high yields of highly intact DNA.

Performance data:

High yield (370-800μg/10mL blood)
High purity (A260/A280 > 1.8)
High MWt DNA (>50kb)
Economical large volume sample extraction
Fully scalable (0.3mL to 50mL blood and 5mg to 500mg tissue)
Suitable for recovery of usable yields of DNA from aged blood

RNA/miRNA

We have proven experience in RNA/miRNA isolation from a number of different sample types using validated procedures such as TriZol, Qiagen, PAXgene, MagMax, ReliaPrep, OMEGA extraction kits. Sample types include:

Formalin Fixed Paraffin Embedded Tissue (FFPE)
Whole blood (including samples collected into PAXgene/Tempus tubes)
Leukocytes collected into LeukoLOCK™ tubes
Plasma and sera
Saliva
Frozen tissue and biopsy material
Cultured cells (mammalian and bacterial)

We also offer a number of associated QC options including agarose gel electrophoresis and Bioanalyzer analysis. Our RNA extraction services also include a rapid extraction service.

Quantification and Quality Assessment

We offer a range of nucleic acid quantification options:

PicoGreen assay (DNA)
RiboGreen assay (RNA)
Spectrophotometry/absorbance measurement (DNA and RNA)
qPCR (RNaseP Analysis)

We also offer a number of associated qualitive analysis options including:

Bioanalyzer analysis for DNA, RNA and miRNA
Fragmentation analysis by agarose gel electrophoresis
Purity ratio assessment by OD measurement

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