Blood Group Genotyping

An exciting new range of real-time PCR-based products…

ReadyPlex™ – Rapid genotyping for blood grouping, Platelet (HPA), Neutrophils (HNA) and coagulation factors

(Currently in development and looking for commercialisation partners)

Real-time PCR blood group genotyping

The Biofortuna ReadyPlex™ product family is an exciting new range of real-time PCR-based products for genomic analysis of erythrocyte blood groups, Human Platelet Antigens (HPA), Human Neutrophil Antigens (HNA) and coagulation polymorphisms. The kits are ready to go – just add DNA to the ReadyPlex™ assay and amplify. After PCR, the ReadyPlex Clarity™ software enables easy genotyping.

ReadyPlex workflow

Biofortuna’s ReadyPlex kits analyze genomic DNA. Each PCR reaction has an internal positive control to verify successful amplification allowing confident allelic typing.

HemoPlex™ RBC genotyping

A series of modular kits that can be used in combination to define a range of blood group genotypes, including the commonly requested RHD, RHCE, Kell, Kidd and Duffy polymorphisms.

Product Number Product Name Specification Format Tests per Kit
HP1 HemoPlex RHD Screening Kit Determination of D+/- status: RHD exons 1, 2, 3, 4 & 7 and detection of RHD psi allele 8 Reactions 60
HP2 HemoPlex RHCE Screening Kit RHCE C, c, E, e, Cw,VS+/- 8 Reactions 60
HP4 HemoPlex RHD & CE Genotyping Kit Genotyping both RHD and RHCE exons 1-10 identifying multiple alleles groups, variants and RHD CE recombinant variants 48 Reactions 10
HP6 HemoPlex RHCE Variant Genotyping Kit RHCE C, c, E, e, Cw, Cx, V+/-, VS+/-, (C)ces and various variants 24 Reactions 20
HP7 HemoPlex Kell Genotyping Kit K, k, Kw, Kpa, Kpb, Kp c, Jsa, Jsb 8 Reactions 60
HP8 HemoPlex Kidd & Duffy Genotyping Kit Fya, Fyb, Fya-b, Fyx, Jka, Jsb 8 Reactions 60
HP10 HemoPlex MNS Genotyping Kit M, N, S, s, U, U- 16 Reactions 30
HP11 HemoPlex RHD Variant Genotyping Kit Determination of D+/- status: RHD exons 1 to 10 and detection of RHD psi allele, D weak types 1-6 & 15, various partial RHD 24 Reactions 20
HP27 HemoPlex Multi Blood Group Genotyping Kit Cartwright, Colton, Cromer, Diego, Dombrock, Duffy, Indian, Kidd, Knops, LW, Lutheran, OK, Scianna, Vel 32 Reactions 15

ThromboPlex™ platelet genotyping

A set of modular kits that can be used for genotyping common HPA-1 to 5, 12 and 15 mutations, or a comprehensive test for all 29 HPA alleles.

Product Number Product Name Specification Format Tests per Kit
TP1 ThromboPlex HPA Screening Kit HPA-1a/b, 2a/b, 3a/b, 4a/b, 5a/b, 12aw/bw, & 15a/b 8 Reactions 60
TP3 ThromboPlex HPA Genotyping Kit HPA-1a/b to 29bw 32 Reactions 15

NeutroPlex™ neutrophil genotyping

Kits for HNA-1, 3, 4 and 5 screening.

Product Number Product Name Specification Format Tests per Kit
NP1 NeutroPlex HNA Genotyping Kit Defines A & B alleles of HNA1, 3, 4, 5 8 Reactions 60

CoaguPlex™ coagulation factor genotyping

Kits for Factor V Leiden, Factor II G20210A and MTHFR (methylenetetrahydrofolate reductase) C677T.

Product Number Product Name Specification Format Tests per Kit
CG1 CoaguPlex Genotyping Kit 1691G>A Factor V Leiden polymorphism,
prothrombin (factor II) 20210G>A, MTHFR C677T
8 Reactions 60

Please enquire for other blood group antigens, kit combinations or polymorphisms

For Investigational Use Only. Products currently in development.

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